Gapmer ASO Designer - Yunnan University

Gapmer ASO Design Submission

Gapmer ASO Mechanism

Antisense oligonucleotides (ASOs) bind to target RNA through complementary base pairing. This binding event recruits RNase H1 enzyme, which cleaves the target RNA, thereby preventing protein translation and reducing target gene expression.

Antisense oligonucleotides (ASOs) bind to the target RNA via complementary base pairing

Recruit intracellular RNase H1 enzyme

RNase H1 catalyzes hydrolysis of the RNA strand in the RNA-DNA heteroduplex

Degradation of target RNA blocks protein translation

About Gapmer ASO Designer

Platform Overview

Gapmer ASO Designer is an Antisense Oligonucleotide (ASO) design platform developed by the Bioinformatics team at Yunnan University. The platform utilizes advanced algorithms to provide high-efficiency, precise ASO design solutions.

Technical Mechanism

Leveraging sequence characteristics, secondary structures, and epigenetic information of target genes in combination with machine learning models to predict optimal ASO binding sites, enhancing silencing efficiency and specificity.

Analysis Pipeline

1. Gene Sequence Acquisition → 2. Target Site Prediction → 3. Specificity Validation → 4. Off-target Effect Assessment → 5. Result Optimization → 6. Report Generation

Applications

Gene function research, drug discovery, genetic disease therapy, cancer targeted treatments, and other biomedical research domains.