About Clin ASO Designer
Platform Overview
Gapmer ASO Designer is an Antisense Oligonucleotide (ASO) design platform developed by the Bioinformatics team at Yunnan University. The platform utilizes advanced algorithms to provide high-efficiency, precise ASO design solutions.
Analysis Pipeline
1. Gene Sequence Acquisition → 2. Target Site Prediction → 3. Specificity Validation → 4. Off-target Effect Assessment → 5. Result Optimization → 6. Report Generation
Gapmer ASO Mechanism
reference: Roberts, T.C., Langer, R. & Wood, M.J.A. Advances in oligonucleotide drug delivery. Nat Rev Drug Discov 19, 673–694 (2020). https://doi.org/10.1038/s41573-020-0075-7
Antisense oligonucleotides (ASOs) bind to target RNA through complementary base pairing.
This binding event recruits RNase H1 enzyme, which cleaves the target RNA,
thereby preventing protein translation and reducing target gene expression.
Antisense oligonucleotides (ASOs) bind to the target RNA via complementary base pairing
Recruit intracellular RNase H1 enzyme
RNase H1 catalyzes hydrolysis of the RNA strand in the RNA-DNA heteroduplex
Degradation of target RNA blocks protein translation
Gapmer ASO Design Submission
Investigate ASO Homology
Investigating the compatibility of ASO across different species (Based on RNAhybrid v2.12).
Investigate SNP in ASO
SNP157
Off-Target Effect Analysis
Citation & Contact Information
Developer & Contact
Principal Investigator
Yunkun Dang,Fan Lai
School of Life Sciences
Yunnan University
ShunKai Chen
School of Life Sciences
Yunnan University
chenshunkai@stu.ynu.edu.cn
Phone
+86 18725174715
Address
School of Life Sciences
Yunnan University
Kunming, Yunnan Province, China
Available Tools
ASO Design Tool
Design gapmer ASOs with optimized parameters
Homology Analysis Tool
Analyze ASO compatibility across species
SNP Analysis Tool
Evaluate SNP effects on ASO binding
Off-Target Analysis Tool
Predict potential off-target effects